Journal: Stroke

Article Title: Severe Blood–Brain Barrier Disruption and Surrounding Tissue Injury

doi: 10.1161/strokeaha.109.551341

Figure Lengend Snippet: Figure 3. Ultrastructural localization of high-molecular-weight dextran–FITC. The localization of fluorescence (A) was confirmed using anti-FITC and a secondary reporter imaged with diaminobenzidine (B). Using FITC fluorescence on vibratome sections, we selected blocks from regions of high dextran accumulation, low dextran accumulation, and homologous sections from the contralateral sides. Ultrastructural examination at 5000 magnification revealed significant vascular labeling of the endothelial cells in regions of high- dextran fluorescence (C) and far less labeling in regions of low-dextran fluorescence (D) or the contralateral side (E). Higher-power views of the selected regions of interest are shown in C*, D*, and E*, respectively. C* demonstrates label contained in endothelial cells and some gross poration of the cell membrane.

Article Snippet: Bound fluorescein was visualized by incubation of sections with biotinylated antifluorescein antibody (BA-0601; Vector; 1:1000 dilution) for 1 day followed by peroxidase catalysis of diaminobenzidine reporter (ABC kit PK6100 Vector and diaminobenzidine kit, SK4100; Vector).

Techniques: High Molecular Weight, Labeling, Membrane